This test is an analysis of cerebral spinal fluid (CSF). CSF bathes, cushions, and protects the brain and spinal cord. It flows around the skull and spine in the subarachnoid space (the area immediately surrounding the brain and spinal cord).

CSF chemistry test identifies chemicals such as proteins and glucose levels, which can help diagnose certain disorders and diseases.

A lumbar puncture (spinal tap) is the most common means of CSF collection. Generally, this is performed as follows: You are positioned on your side with your knees curled up to your abdomen and your chin tucked in to your chest. Local anesthetic is injected over the lower spine. The spinal needle is inserted, usually between the 3rd and 4th lumbar vertebrae, and fluid is withdrawn for testing. You will be asked to remain flat, or nearly flat, for 6 to 8 hours after the test.

Alternative methods of obtaining CSF are rarely used, but they may be recommended if you have a problem such as lumbar deformity or infection, which would make lumbar puncture impossible or unreliable:

• Cisternal puncture involves insertion of a needle below the occipital bone (back of the skull). This procedure can be hazardous because the needle is inserted close to the brain stem.
• Ventricular puncture is even more rare, but may be indicated when sampling of CSF is necessary in people with possible impending brain herniation. It is usually performed in the operating room. A hole is drilled in the skull and a needle is inserted directly into the lateral ventricle of the brain.

The CSF is then sent to the laboratory for evaluation.

Be prepared to sign a consent form and to remain in the hospital for at least 6 to 8 hours after the test.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:

• Infant test or procedure preparation (birth to 1 year)
• Toddler test or procedure preparation (1 to 3 years)
• Preschooler test or procedure preparation (3 to 6 years)
• Schoolage test or procedure preparation (6 to 12 years)
• Adolescent test or procedure preparation (12 to 18 years)

The curled position may be uncomfortable but is necessary to avoid moving the needle and possibly injuring the spinal cord.

The anesthetic will sting or burn when first injected. There will be a hard pressure sensation when the needle is inserted, and there is usually some brief pain when the needle goes through the meninges. (See CSF collection.)

Overall, discomfort is minimal to moderate. The entire procedure usually takes about 30 minutes. The actual fluid collection only takes a few minutes.

• CSF cell count
• CSF culture
• CSF total protein
• CSF glucose
• CSF VDRL test (syphilis serology)
• CSF coccidioides complement fixation (fungus)
• CSF oligoclonal banding (immunofixation)
• Glutamine
• Lactate dehydrogenase
• Chloride
• Cytology

• Cell count: less than 0 to 5 white blood cells (WBC) and 0 red blood cells (RBC)
• Culture and sensitivity: no organism growth
• Protein: 15 to 45 mg/dL
• Glucose: 50 to 80 mg/100 mL
• Serology for syphilis: no presence of antibodies
• Fungus: no presence of fungus
• Immunofixation: one or fewer bandings
• Glutamine: 6 to 15 mg/dL
• Lactate dehydrogenase: less than 2.0 to 7.2 U/mL
• Chloride: 700 to 750 mg/dL
• Cytology: no malignant cells present

Abnormal glutamine results may indicate hepatic encephalopathy or Reye's syndrome. Elevated lactate dehydrogenase levels may indicate inflammation or infection. Decreased glucose may suggest meningitis due to bacteria, fungus, or tuberculosis. Increased white blood cells may indicate an infection or another inflammatory process.

For more information on abnormal values, please see the specific test.

Risks of lumbar puncture include:

• Hypersensitivity (allergic) reaction to the anesthetic
• Discomfort during the test
• Headache after the test
• Bleeding into the spinal canal
• Brain herniation (if performed on a person with increased intracranial pressure), which can cause brain damage and/or death
• Damage to the spinal cord or nerve roots (particularly if the person moves during the test)
• Infection following a nonsterile procedure (very rare)

Cisternal puncture or ventricular puncture carries additional risk of damage to the brainstem or brain tissue and risk of bleeding within the brain, potentially resulting in incapacitation or death.