Blood is drawn from a vein, usually on the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and an elastic band is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the band to fill with blood.

A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

For an infant or young child, the area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.

The sample is taken to the laboratory and evaluated for the presence and quantity of antibodies.

• infant test or procedure preparation (birth to 1 year)
• toddler test or procedure preparation (1 to 3 years)
• preschooler test or procedure preparation (3 to 6 years)
• schoolage test or procedure preparation (6 to 12 years)
• adolescent test or procedure preparation (12 to 18 years)

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

This test is done to detect if you have been infected with the herpes simplex virus (1 or 2). This test does not detect the virus itself. If antibodies to the virus are present, you have been infected with herpes simplex at some point in your life.

If the infection is very recent (few weeks to month), antibodies may not be detected (window period), but you may still be infected.

The presence of antibodies indicates that you have been infected with herpes-simplex recently or at some point in the past. Certain patterns of antibodies can sometimes help determine whether the infection is recent.

If you have been infected within the past weeks to month, your body may not have yet mounted an antibody response and the test may be falsely negative (window period).

Herpes simplex virus stays in your system once you have been infected. It may be dormant and cause no symptoms, or intermittently reactivate (flare-up) and cause symptoms. This test is not helpful in determining whether you are having a flare or reactivation.

HSV-2 is commonly associated with genital herpes while HSV-1 is usually associated with cold sores (oral herpes), however, some cases of genital herpes are caused by HSV-1.

• excessive bleeding
• fainting or feeling lightheaded
• hematoma (blood accumulating under the skin)
• infection (a slight risk any time the skin is broken)
• multiple punctures to locate veins